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P25 VINCLOZOLIN, BISPHENOL A, GENISTEIN AFFECT EXPRESSION OF NUCLEAR VITAMIN D3 RECEPTOR AND CYP24 AND CYP27B1 IN HUMAN MCF-7 CELLS Slavomra Ondkov, Dana Macejov, Mria Fickov, Jlius Brtko Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlrska 3, 83306 Bratislava, Slovak Republic The following results were obtained using the human MCF-7 cell line treated with the above compounds at the concentration of 1 mol l for 24 h and 72 h. Semiquantitative RT-PCR analysis was used for the determination of relative mRNA expression levels of VDR as well as vitamin D3 synthesizing enzyme 1-hydroxylase CYP27B1 ; and degrading enzyme 24-hydroxylase CYP24 ; . In conclusion, vinclozolin was found to enhance expression of CYP27B1 and to decrease expression of CYP24 after both 24 h or treatment. BPA caused enhanced expression of VDR and CYP27B1 after 24 h treatment. Genistein treatment of the MCF-7 cells for 72 h resulted in decreased expression of CYP24. These results from in vitro experiments suggest that these compounds may play a marked role in modulation of nuclear vitamin D3 receptor expression and also in expression of 1-hydroxylase and 24-hydroxylase in MCF-7 cells. This work was supported by the grant of the European Commission No.: FOOD-CT-2004-506319 and the VEGA grant No.2 5017 5.

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Subcommittee members: Bret Lashner, M.D. Cleveland Clinic Foundation Teresa Brentnall, M.D. University of Washington Robert Riddell, M.D. University of Toronto Steven Itzkowitz, M.D. Mount Sinai School of Medicine Dawn Provenzale. M.D. Duke University David Rubin, M.D. University of Chicago. Walker MP, Ayre GA, Ashton CH, Marsh VR, Wesnes K, Perry EK, O'Brien JT, McKeith IG, Ballard CG. 1999 ; . A psychophysiological investigation of fluctuating consciousness in neurodegenerative dementias. Human Psychopharmacology 14: 483-489. Walker MP, Ayre GA, Cummings JL, Wesnes K, McKeith IG, O'Brien JT, Ballard CG. 2000 ; . The Clinician Assessment of Fluctuation and the One Day Fluctuation Assessment Scale. British Journal of Psychiatry 177: 252-256. Walker MP, Ayre GA, Perry EK, Wesnes K, McKeith IG, Tovee M, Edwardson JA, Ballard CG 2000 ; . Quantification and Characterisation of fluctuating cognition in dementia with Lewy Bodies and Alzheimer's disease. Dementia and Geriatric Cognitive Disorders 11: 327-335.

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The subsidiaries held or acquired exclusively with a view to subsequent resale within twelve months from acquisition date for which management is actively seeking a buyer are excluded from consolidation and are included as available-for-sale investments and measured at fair value where this can be reliably measured or at cost less impairment losses where fair value cannot be reliably measured. All material inter-company accounts and transactions have been eliminated in consolidation. The equity and net income attributable to minority interests are shown as separate items in the consolidated financial statements. 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Control male reproductive system Male exposed to 33 g cyproterone acetate with two previtellogenic oocytes pvoc ; Male exposed to 3.5 ng l DDE with apoptotic spermatogonia, spermatocytes and spermatids and extended intercellular spaces in the gonad Male exposed to 3.5 ng l triphenyltin spermatophore with unilateral hypertrophied spermatophore wall and pale core secretions Male exposed to 0.24 mg l vinclozolin with slightly vacuolated spermatocytes arrow ; and deformed spermatophore wall dotted arrow ; H.
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